Effects of Adipose-Derived Mesenchymal Stem Cells and Human Amniotic Membrane on Sciatic Nerve Repair in Rats

Background: Peripheral nerve injuries remain a great challenge for microsurgery despite the significant progress in recent decades. The current gold standard is autogenous grafting with success rate as low 50% long gaps. Current studies have focused on finding alternative methods bridging defects. Previous data demonstrated role of human amniotic membrane stimulating neural regeneration. On other hand, adipose-derived mesenchymal stem cells can differentiate into all three germ layers and could support repair. purpose this study was to compare without adipose tissue sciatic injury gap rats. Objectives: We aimed evaluate effectiveness Methods: Twenty-four male Wistar rats four random groups were used our study. In first group, repaired using inverse resected segment (Control group), second group (AM third an amnion sheet seeded (AM/ADMSCs last not repaired, both stumps sutured muscles. Results: All animals underwent procedures survived complication. function index hot plate test results significantly improved AM AM/ADMSCs compared Control (as care) (P>0.05). Based histopathology findings, regenerative fibers seen implanted area groups; however, surrounded by fibrosis (scar formation) group. axon count higher than experimental (P < 0.01). Conclusions: Our showed promotion regeneration gap, but adding only has no extra benefits, also causes more scar.